Porcine Acute Diarrhea Syndrome Coronavirus (SADS-CoV) ichangobva kuwanikwa porcine enteric pathogenic coronavirus inogona kukonzera manyoka emvura munguruve dzichangozvarwa uye kukonzera kurasikirwa kukuru kwehupfumi kuindasitiri yenguruve.Parizvino, hapana yakakodzera serological nzira yekuongorora kushanda kweSADS-CoV utachiona uye vaccine, saka pane kudiwa kwekukurumidzira kushandisa inoshanda enzyme-yakabatana immunosorbent assay (ELISA) kugadzirisa kushomeka uku.Pano, recombinant plasmid inoratidzira SADS-CoV spike (S) protein yakasanganiswa nemunhu IgG Fc domain yakavakwa kuti ibudise recombinant baculovirus uye inoratidzwa muHEK 293F maseru.Iyo S-Fc protein inocheneswa neprotein G resin uye inochengeta reactivity ine anti-munhu Fc uye anti-SADS-CoV masoja ekudzivirira chirwere.Ipapo S-Fc protein yakashandiswa kugadzira isina kunanga ELISA (S-iELISA) uye kugadzirisa maitiro eS-iELISA.Nekuda kweizvozvo, nekuongorora iyo OD450nm kukosha kwe40 SADS-CoV isina kunaka sera yakasimbiswa ne immunofluorescence assay (IFA) uye Western blotting, kukosha kwekucheka kwakatemwa kuve 0.3711.Iyo coefficient of variation (CV) ye6 SADS-CoV yakanaka sera mukati uye pakati pekumhanya kweS-iELISA ese aive asingasviki gumi%.Muchinjika-reactivity bvunzo yakaratidza kuti S-iELISA haina muchinjiko-reactivity neimwe porcine virus sera.Mukuwedzera, zvichibva pakuonekwa kwe 111 kliniki serum samples, iyo yose inopindirana mwero weIFA neS-iELISA waiva 97.3%.Hutachiona hwekunerera kuita bvunzo ine 7 dzakasiyana OD450nm kukosha kweserum yakaratidza kuti iyo OD450nm kukosha yakaonekwa neS-iELISA yaive yakabatana nehutachiona neutralization bvunzo.Pakupedzisira, S-iELISA yakaitwa pa300 yenguruve purazi serum samples.Commerce kits yedzimwe porcine enteroviruses yakaratidza kuti IgG yakanaka mitengo yeSADS-CoV, TGEV, PDCoV uye PEDV yaive 81.7%, 54%, uye 65.3%, zvichiteerana., 6%, zvichiteerana.Mhedzisiro yacho inoratidza kuti iyo S-iELISA yakanangana, ine hanya, uye inoberekazve, uye inogona kushandiswa kuona hutachiona hweSADS-CoV muindasitiri yenguruve.Ichi chinyorwa chinochengetedzwa nekodzero.kodzero dzose dzakachengetedzwa.
Nguva yekutumira: Jun-22-2021